Donovan, who plays for the Los Angeles Galaxy, cited irreconcilable differences for the split with Kajlich after nearly three years of marriage.
Kajlich currently stars in the CBS comedy "Rules of Engagement."
They were married on New Year's Eve 2006 and have no children together. He states the couple separated in October 2009.
In a statement, the former couple says, "We remain the closest of friends and will always be important parts of each others lives."
Donovan was a key member of the United States' 2010 World Cup team, scoring a goal against Algeria that advanced the team to the second round of play.