Three hundred works of fine art and memorabilia that belonged to Hopper are up for bids.
Most of the items were in Hopper's Venice Beach home.
Among the highlights is an Andy Warhol portrait of Mao Zedong that Hopper shot two bullet holes into.
There will also be posters from Hopper's movies.
Hopper, a photographer and painter himself, became immersed in the West Coast artist scene and pop art movement, becoming close friends with Warhol, Roy Lichtenstein and many of the other artists he collected.
The sale does not include Hopper's own photography and paintings.
Hopper, who was twice nominated for Oscars and earned a star last year on the Hollywood Walk of Fame, died of prostate cancer at his home in May. He was 74.
The Associated Press contributed to this report.