It happened around 1:20 am on Monday.
The California Highway Patrol says 39-year-old Carson Enns was traveling on Copper Avenue toward Peach Avenue just outside Fresno.
Investigators say the intersection is currently closed due to road work in the area, and the road has several construction signs leading up to it.
For some reason, Enns kept going toward the intersection and suddenly tried to brake to avoid hitting a tractor, officials said.
The bike slid and overturned, hitting a construction sign and colliding with the tractor.
The CHP says Enns was thrown from the bike. He died at the scene.
The crash is still under investigation. Officers say it's unclear at this point if alcohol or drugs were factors in the accident.