The run started in Fresno at Woodward Park and moved along Shepherd and Friant Avenue to Willow and down into Old Town Clovis.
Several of the main roads in the area were closed until the end of the race.
This year, a group of participants ran for Neng Thao.
The 18-year-old senior at Edison High School drowned in the San Joaquin River back in May. Thao was a longtime Two Cities volunteer and planned to run this year's half marathon.