The Fresno County Sheriff's Office confirmed that 39-year-old Natalie Shepherd and two-year-old Savanah Fagundes were killed in the crash. Deputies say Fagundes was Sheperd's daughter.
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The California Highway Patrol says the crash happened at about 2:40 pm on Highway 33 north of Sutter Avenue.
Investigators say a pickup truck and a van collided on the southbound lane of the highway.
The truck caught fire was quickly consumed by the flames.
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Officers are still working to determine what led up to the crash.
The third person killed has not been identified.