The shooting happened just after 1 am Sunday at a home near Lane and Backer.
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First responders performed CPR on 31-year-old Felicia Becerra, but she died at the scene, on her birthday.
Oudara Chounramany, 29, was rushed to the hospital, where he was later pronounced dead.
A third shooting victim showed up at the same hospital, but that 26-year-old man is expected to recover.
Police spent the morning interviewing witnesses but they say the majority of people who were at the party haven't been cooperating.
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Officials say the party was actually Becerra's birthday celebration.
There's no word yet on possible suspects or a motive.
Fresno has now had 11 homicides since the start of the year.