Officers responded to Shotspotter report of seven shots being fired at Kearney Boulevard and Thorne Avenue before 11 pm Tuesday.
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When investigators arrived, they found a woman inside a car with a gunshot wound.
She was rushed to Community Regional Medical Center, where she was last reported in critical condition.
A motive for the shooting has not been determined. Police said they have a witness helping them in their investigation.
No suspect information has been released.
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Officers spent the early morning hours Wednesday canvassing the area looking for more evidence.
They're asking anyone with information about the shooting to call the Fresno Police Department.