A 2-year-old filly named Bye Bye Beautiful injured its leg during a race Sunday and was euthanized.
Another horse died Friday.
Gov. Gavin Newsom signed a law in June allowing the agency overseeing horse racing in California to immediately suspend licenses to protect the health and safety of horses and riders as a response to the rise in horse deaths at the park.
The race track has seen a significant drop in attendance since the controversial horse deaths.
Santa Anita Park is scheduled to host the Breeders' Cup which starts Friday.