Deputies on horseback accepted donations from the community at the corner of Van Ness and Griffith.
That's the same intersection where a teenager was stabbed on December 13.
Detectives believe the stabbing was gang-related.
The incident happened as families strolled down the two-mile, holiday display on Van Ness Boulevard.
Fresno County Sheriff Margaret Mims said she hopes a single violent event can create a lasting change.
"Christmas Tree Lane is kind of a sacred place for Fresno. This is a place where people come, families come, kids come and we don't expect and neither do we want violence here. So this is a statement that we're making. We're not going to allow it," Mims said.
The sheriff says she was impressed with the donations they received Thursday night.
One generous donor wrote a $500 check and a few others dropped hundred dollar bills into the bag.